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Detection of point mutations in the chloroplast genome by single-stranded conformation polymorphism analysis

✍ Scribed by Kin-Ying To; Chiu-I. Liu; Shih-Tung Liu; Yu-Sun Chang

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 465 KB
Volume: 3
Category: Article
ISSN: 0960-7412
DOI: 10.1046/j.1365-313x.1993.t01-10-00999.x

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✦ Synopsis

Summary

Single‐stranded conformation polymorphism (SSCP) analysis was used to examine the mutations of the chloroplast 16S rRNA locus of streptomycin‐resistant mutants in Nicotiana plumbaginifolia. DNA fragments of 121, 517, 968 and 1578 bp, each possessing a known point mutation, were generated by polymerase chain reaction (PCR). The resulting fragments were denatured and separated by nondenaturing polyacrylamide gel electrophoresis. Compared to the patterns of the wild‐type DNA fragments, the bands of the single‐stranded DNA fragments of 121 and 517 bp with base changes were shifted. However, no pattern variations were detected from the DNA fragments of 968 and 1578 bp generated from both wild‐type and mutants.

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