✦ LIBER ✦

Detection of Paracoccidioides brasiliensis gp43 Gene in Sputa by Loop-Mediated Isothermal Amplification Method

✍ Scribed by B. T. Tatibana; A. Sano; J. Uno; K. Kamei; T. Igarashi; Y. Mikami; M. Miyaji; K. Nishimura; E. N. Itano

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 121 KB
Volume: 23
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.20304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The fungus Paracoccidioides brasiliensis is the pathogen of paracoccidioidomycosis (PCM), a systemic mycosis prevalent in Latin America. The loop‐mediated isothermal amplification method (LAMP) was used in this study to detect the presence of P. brasiliensis in sputa samples from patients with chronic PCM, suspected PCM, and a negative control. The target P. brasiliensis gp43 gene was amplified in less than 4 hr in 11 of 18 sputa samples tested. The LAMPmethod had the advantage of speed and simplicity compared with the classic diagnostic methods such as the histopathological test or biological material culture and did not require sophisticated technical apparatus. It would be an important aid in cases where immediate treatment would mean patient survival, especially in immune‐suppressed patients. J. Clin. Lab. Anal. 23:139–143 2009. © 2009 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Rapid detection of varicella–zoster viru

Rapid detection of varicella–zoster virus infection by a loop-mediated isothermal amplification method

✍ Souki Okamoto; Tetsushi Yoshikawa; Masaru Ihira; Kayoko Suzuki; Kaoru Shimokata; 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 203 KB

## Abstract The reliability of varicella–zoster virus (VZV) loop‐mediated isothermal amplification (LAMP) was evaluated for rapid diagnosis of viral infection. VZV‐specific primers only amplified VZV DNA; no LAMP products were observed in reactions performed with other viral DNA templates. The spec

A simple method for the detection of mea

A simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP)

✍ Motoko Fujino; Naoko Yoshida; Shinya Yamaguchi; Norimitsu Hosaka; Yoshinori Ota; 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 381 KB

Approximately 20,000-30,000 measles patients were reported in a surveillance of infectious diseases because of low vaccine coverage of 80% in Japan. Among them, some were thought to be secondary vaccine failure (SVF) with generally mild or non-typical measles illness and sometimes became a source of