✦ LIBER ✦

Detection of novel non-ras oncogenes in rat nasal squamous cell carcinomas

✍ Scribed by Anne E. Hochwalt; Isaac Wirgin; Mary Felber; Diane D. Currie; Seymour J. Garte

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 316 KB
Volume: 1
Category: Article
ISSN: 0899-1987
DOI: 10.1002/mc.2940010104

No coin nor oath required. For personal study only.

✦ Synopsis

Rat nasal squamous cell carcinomas induced by inhalation of three direct-acting alkylating agents yielded DNA containing activated oncogenes with no homology t o any member of the ras family. The novel NIH 3T3 transforming oncogenes from tumors induced by p-propiolactone and methylmethane sulfonate are distinct from each other based on restriction analysis. The gene isolated from p-propiolactone-induced tumors is between 6 and 9 kb in size. None of the tumors induced by dimethylcarbamyl chloride contained positive DNA in the NIH 3T3 focus assay or in the nude mouse cotransfection assay. The rat nasal tumor model is apparently ideally suited for analysis of the roles of carcinogen and tissue specificity in oncogene activation, especially related t o novel (non-ras) transforming oncogenes.

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