Detection of non-covalent protein interactions by 'intensity fading' MALDI-TOF mass spectrometry: applications to proteases and protease inhibitors

Rapid methods for screening low molecula

Rapid methods for screening low molecular mass compounds non-covalently bound to proteins using size exclusion and mass spectrometry applied to inhibitors of human cytomegalovirus protease

✍ Siegel, Marshall M.; Tabei, Keiko; Bebernitz, Geraldine A.; Baum, Ellen Z. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 650 KB

General and rapid methods were developed for determining the extent of non-covalent binding between small molecules and proteins, using the model system of human cytomegalovirus protease and several drug candidates which inhibit the protease by non-covalently binding to it. The assay was performed b