✦ LIBER ✦
Detection of neutral amino acid mutations by immobilized pH gradients: The case of the Tγ variant in fetal hemoglobin Sardinia
✍ Scribed by Dr. Gianfranco Cossu; Mario Manca; Pier Giorgio Righetti; Elisabetta Gianazza; VÉRonique Baudin; Henri Wajcman; Adriana Bianchi-Bosisio
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 433 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Detection of neutral amino acid mutations by immobilized pH gradients: The case of the Ty variant in fetal hemoglobin Sardinia
A method for separating fetal hemoglobin Sardinia (AyT, a variant containing threonine in position 75 instead of isoleucine) from normal fetal hemoglobin is described, based on the use of shallow immobilized pH gradients (pH 7.30-7.55). The novelty of the fractionation technique is the direct analysis of intact, native hemoglobin tetramers, while present methods utilize only reverse-phase, high performance liquid chromatography of denatured, heme-free globin chains.