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Detection of Neorickettsia (Ehrlichia) risticii in tissues of mice experimentally infected with cercariae of trematodes by in situ hybridization

✍ Scribed by Joon-seok Chae; Min-seok Kim; John Madigan

Book ID: 114245507
Publisher: Elsevier Science
Year: 2002
Tongue: English
Weight: 283 KB
Volume: 88
Category: Article
ISSN: 0378-1135
DOI: 10.1016/s0378-1135(02)00112-8

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## Abstract In situ hybridization has been used as a quantitative method to study the replication of SV40 during lytic infection. Various parameters have been defined such as fixation and denaturation of cytological preparations, concentration of complementary (^3^H)‐cRNA and volume of buffer used