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Detection of naturally occurring enteroviruses in waters using direct RT-PCR and integrated cell culture-RT-PCR

✍ Scribed by Y.C. Shieh; C.I. Wong; J.A. Krantz; F.C. Hsu


Book ID
113767736
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
131 KB
Volume
149
Category
Article
ISSN
0166-0934

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Reverse transcription-polymerase chain reaction (RT-PCR) methods are available for the rapid detection of enteroviruses in clinical specimens or virus isolates. Pan-enterovirus PCR primers, however, fail to amplify echovirus (E) type 22 or 23 because of their extreme sequence divergence from the oth