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Detection of mutations and polymorphisms in a candidate male fertility gene by screening infertile patients.

โœ Scribed by E.Y Xu; D Lee; P.J Turek; R.A Reijo Pera


Book ID
119417600
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
21 KB
Volume
76
Category
Article
ISSN
1556-5653

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Objective: We used single-,'~trand conformational polymorphism (SSCP) to screen for mutations at nucleotides 833 and 919 of the cystathionine ~-synthase (CBS) gene in 13 patients with homocystinuria and 11 of their relatives. Methods: Exon 8 of genomic DNA was selectively amplified by PCR using pri