Detection of mumps virus genome directly from clinical samples and a simple method for genetic differentiation of the Hoshino vaccine strain from wild strains of mumps virus
✍ Scribed by Kashiwagi, Yasuyo; Kawashima, Hisashi; Takekuma, Kouji; Hoshika, Akinori; Mori, Takayuki; Nakayama, Tetsuo
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 486 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
A simple and sensitive method was developed for the differentiation of the Hoshino vaccine strain from wild strains with a restriction fragment length polymorphism (RFLP) analysis in the part of hemagglutinin-neuraminidase (HN) gene. The virus genome was amplified by using a reverse transcriptase-polymerase chain reaction (RT-PCR) directly from clinical samples. The PCR product of the Hoshino vaccine strain was cleaved into 2 fragments after digestion with Sca I and Afl II. All wild strains showed 2 RFLP profiles, A and B, different from that of vaccine strain. Wild A strains were cut into 2 fragments after digestion with Sca I only, while wild B strains were cleaved neither with Sca I nor Afl II. This molecular approach provides an effective method for differentiation of the Hoshino vaccine strain from wild strains of mumps virus in patients after vaccination.