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Detection of Multidrug Resistance (MDR1) Gene RNA Expression in Human Tumors by a Sensitive Ribonuclease Protection Assay

โœ Scribed by Ueda, Kazumitsu ;Yamano, Yoshiaki ;Kioka, Noriyuki ;Kakehi, Yoshiyuki ;Yoshida, Osamu ;Gottesman, Michael M. ;Pastan, Ira ;Komano, Tohru

Book ID
108578912
Publisher
Wiley (Blackwell Publishing)
Year
1989
Tongue
English
Weight
441 KB
Volume
80
Category
Article
ISSN
0910-5050

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Quantitative analysis of human multidrug
Quantitative analysis of human multidrug resistance 1 (MDR1) gene expression by nonisotopic competitive reverse transcriptase polymerase chain reaction assay
โœ Hiroyuki Kobayashi; Yuzuru Takemura; Hayato Miyachi; Masaki Kawabata; Shigehisa ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 124 KB ๐Ÿ‘ 2 views

We have established competitive reverse transcriptase polymerase chain reaction (RT-PCR) assay for the quantification of MDR1 mRNA encoding P-glycoprotein (P-gp) by analyzing leukemia sublines of MOLT-3 with various expression of MDR1. The expression was quantified by simultaneous RT-PCR of cellular