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Detection of mRNAs encoding peroxisomal proteins by non-radioactive in situ hybridization with digoxigenin-labelled cRNAs

✍ Scribed by E. Baumgart; Arno Schad; Alfred Völkl; H. Dariush Fahimi


Publisher
Springer
Year
1997
Tongue
English
Weight
957 KB
Volume
108
Category
Article
ISSN
1432-119X

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Messenger RNAs (mRNAs) encoding procollagen cd type I, cd type II and cd type III have been localized in paraffin sections of human fetal fingers using digoxigenin-labelled synthetic oligonucleotide probes. The probe-mRNA hybrids were visualized using an anti-digoxin antibody amplified with sandwich