Detection of molluscum contagiosum virus gene transcripts by in situ hybridization
β Scribed by Watanabe, Takahiro; Tamaki, Kunihiko
- Book ID
- 119293454
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 344 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0923-1811
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Optimal conditions for the detection of complex RNA sequences in individual cells by in situ hybridization have been determined by using in vitro cultured quail embryonic cells infected with Rous sarcoma virus and a single-stranded 3H-cDNA probe of high specific activity complementary to the RSV gen
A polymerase chain reaction (PCR) which enables the detection of molluscum contagiosum virus (MCV) genomes in either fresh or formalinfixed clinical specimens is described. The primers used were designed to amplify a 167 bp region of the 3.8kbp HindIII fragment K of the MCV 1 genome. The ability of