Detection of Modified Amino Acids in Lantibiotic Peptide Mutacin II by Chemical Derivatization and Electrospray Ionization–Mass Spectroscopic Analysis

✍ Scribed by Jan Novák; Marion Kirk; Page W. Caufield; Stephen Barnes; Kelly Morrison; John Baker

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 68 KB
• Volume: 236
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1996.0181

✦ Synopsis

Washington, DC.

spectroscopy which effectively yields information on the total number of modified amino acids in the lantibiotic mutacin II. Amino acid analysis of nisin Z and its chemically modified variant confirmed the results. Using two different chemical modifications also al-

Detection of Modified Amino Acids in

lowed differentiation between thioether amino acids Lantibiotic Peptide Mutacin II by Chemical and a,b-unsaturated amino acids.

Derivatization and Electrospray Ionization-

Bacterial strains, media, and mutacin assay. The Mass Spectroscopic Analysis mutacin II-producing strain S. mutans T8 and the indicator strain S. sanguis Ny101 (11) were used in this study. The strains were stored frozen at 070ЊC in