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Detection of minimal residual disease in hematologic malignancies by real-time quantitative PCR: principles, approaches, and laboratory aspects

✍ Scribed by van der Velden, V H J; Hochhaus, A; Cazzaniga, G; Szczepanski, T; Gabert, J; van Dongen, J J M


Book ID
110055467
Publisher
Nature Publishing Group
Year
2003
Tongue
English
Weight
572 KB
Volume
17
Category
Article
ISSN
0887-6924

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Because previous PCR-based methodologies for detection of minimal residual disease (MRD) in leukemia patients have been too cumbersome to allow for widespread clinical usefulness, we have employed a real-time quantitative PCR (RQ-PCR) system to develop an MRD assay for t(12;21). We initially determi