Detection of methyltransferase activities which modify Gppp G TO m7GpppGm in embryonic chick lens

✍ Scribed by Gene C. Lavers

• Book ID: 104743499
• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 315 KB
• Volume: 3
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00368297

✦ Synopsis

GpppG was modified to m7 GpppGm by a cytoplasmic extract, prepared from embryonic lens cells, in a reaction mixture which contained S-adenosyl-methionine as methyl group donor. The appearance of m7 GpppGm was a function of time and lens extract concentration. S-adenosyl-homocysteine inhibited both the m7G and Gm modification reactions. Analogues of GpppG, pG, ppG and pppG were relatively ineffective as substrates.