Detection of Metabolites of the Entner-Doudoroff Pathway by HPLC with Pulsed Amperometry: Application to Assays for Pathway Enzymes

Detection of Glycinamide Ribonucleotide

Detection of Glycinamide Ribonucleotide by HPLC with Pulsed Amperometry: Application to the Assay for Glutamine:5-Phosphoribosyl-1-pyrophosphate Amidotransferase (EC 2.4.2.14)

✍ T.S.M Taha; T.L Deits 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 499 KB

An assay method is described for measuring the catalytic activity of glutamine:5-phosphoribosyl-1-pyrophosphate amidotransferase (EC 2.4.2.14), the first enzyme in the de novo purine biosynthetic pathway. Phosphoribosylamine, the unstable product of the enzymatic reaction, is trapped by glycinamide