Detection of menadione sodium bisulfite (vitamin K3) by reversed-phase high performance liquid chromatography with series dual-electrode amperometric detector

✍ Scribed by Zhiming Liu; Tao Li; Jie Li; Erkang Wang

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 500 KB
• Volume: 338
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/s0003-2670(96)00435-7

✦ Synopsis

A reversed-phase high-performance liquid chromatography with series dual glassy carbon electrodes for the amperometric detection of water-soluble menadione is described. The complex post-column derivatization reaction and the high background currents were avoided. The menadione sodium bisulfite was reduced at -0.3 V vs. SCE at the upstream (generator) electrode and oxidized at +0.2 V vs. SCE at the downstream (collector) electrode. The mobile phase was 0.2 mol 1-l HAc-NaAc aqueous buffer (pH 5.50) and 40% (v/v) methanol. The linear response was in the range of 35 ng to 15 pg. with a detection limit of 15 ng (S/%3). The correlation coefficient was 0.9997 (n=6). The electrochemical detection with series dual electrodes has a higher selectivity for menadione (vitamin K1) compound than with UV detection.