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Detection of maternal deoxyribonucleic acid in umbilical cord plasma by using fluorescent polymerase chain reaction amplification of short tandem repeat sequences

✍ Scribed by Margit Bauer; Irmgard Orescovic; Wolfgang M. Schoell; Diana W. Bianchi; Barbara Pertl


Book ID
114487575
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
112 KB
Volume
186
Category
Article
ISSN
1097-6868

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A procedure for amplifying and detecting nucleic acid sequences in situ using cells in suspension and flow cytometry has been developed. The process involves the use of the polymerase chain reaction (PCR) and a fluorescent in situ hybridization (FISH) protocol developed in our laboratory to detect t