✦ LIBER ✦

Detection of mannophosphoinositide antigens in sputum of tuberculosis patients by dot enzyme immunoassay

✍ Scribed by Rita Kansal; G. K. Khuller

Publisher: Springer-Verlag
Year: 1991
Tongue: English
Weight: 502 KB
Volume: 180
Category: Article
ISSN: 0300-8584
DOI: 10.1007/bf00193848

No coin nor oath required. For personal study only.

✦ Synopsis

A simple and economical dot ELISA for the detection of mannophosphoinositide antigen in sputum samples of tuberculosis patients has been developed using affinity-purified antibodies. This test is able to detect free as well as bound antigen. Sputum samples from 94 patients suffering from tuberculosis and 30 non-tuberculosis patients were screened and an overall sensitivity and specificity of 89% and 93.3 %, respectively, was obtained. The sensitivity of the test among the different groups of tuberculosis patients did not vary significantly.

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