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Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides

✍ Scribed by Monika Deo; Jenn-Yah Yu; Kwan-Ho Chung; Melissa Tippens; David L. Turner

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 35 KB
Volume: 236
Category: Article
ISSN: 1058-8388
DOI: 10.1002/dvdy.21079

No coin nor oath required. For personal study only.

✦ Synopsis

The original article included "100 mg/ml heparin" to describe the hybridization buffer, but it should correctly read "100 g/ml heparin." The corrected sentence is published in full below. The authors regret this error.

"Hybridization buffer: 50% formamide, 0.3 mg/ml yeast RNA (ICN), 100 g/ml heparin, 1ϫ Denhardt's solution (0.2% BSA, 0.2% polyvinylpyrrolidone, 0.2% Ficoll 400), 0.1% Tween 20, 0.1% CHAPS, 5 mM EDTA, and 0.3 nmol/ml 12-mer random sequence DNA oligonucleotides."

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