In segregating populations, large numbers of individuals are needed to detect linkage between markers, such as restriction fragment length polymorphisms (RFLPs), and quantitative trait loci (QTL), limiting the potential use of such markers for detecting linkage. Fewer individuals from inbred lines a
Detection of linkage between restriction fragment length polymorphism markers and quantitative traits
โ Scribed by M. J. Asins; E. A. Carbonell
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 339 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0040-5752
No coin nor oath required. For personal study only.
โฆ Synopsis
Methodologies commonly used to detect linkage of marker loci to loci affecting quantitative traits are discussed. It is shown that variances for the quantitative trait differ among marker genotypes when using F2 or pooled backcross data if linkage exists. Hence, to analyze this type of data by single factor ANOVA or other statistical techniques that assume a common variance is inadequate. Restriction fragment length polymorphism (RFLP) markers are a powerful tool in plant breeding but cost is an important drawback; hence, a methodology is suggested to obtain the minimum number of plants in F2 populations to detect such linkage.
๐ SIMILAR VOLUMES
A genetic linkage map of lentil comprising 333 centimorgans (cM) was constructed from 20 restriction fragment length, 8 isozyme, and 6 morphological markers segregating in a single interspecific cross (Lens culinaris ร L. orientalis). Because the genotypes at marker loci were determined for about 66
By making use of pedigree information and information on marker-genotypes of the parent and F-1 individuals crossed to form an F-2 population, it is possible to carry out a linkage analysis between marker loci and loci affecting quantitative traits in a cross between segregating parent populations t