Detection of K-raspoint mutation by enriched PCR–colorimetric plate assay
✍ Scribed by Fernando S Santiago; Alison V Todd; Nicholas J Hawkins; Robyn L Ward
- Book ID
- 115638158
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 406 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0890-8508
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Mutant-enriched PCR was applied to the detection of mutations at codons I 2 and I 3 of K-ras genes in the stools of patients with colorectal cancer. Mutations were analyzed in stool samples obtained prior to surgery. Resected tumor specimens were screened for K-ras mutations by PCR-mediated RFLP ana
We have developed a rapid and highly sensitive method for the detection of mutant K-ras codon 12 allele in the presence of 10 5 copies of the wild-type alleles. This sensitivity is achieved by selective amplification of mutant K-ras sequences, using a two-stage procedure with modified primers. In th