We have studied the interaction of several phosphopeptides with cationic polyamino acids such as polyarginine and polylysine by fluorescence polarization. The phosphopeptides used were labeled with fluorescein, and their net charges at the experimental pH of 7.5 were 0, ุ1, ุ2, and ุ3. These phospho
โฆ LIBER โฆ
Detection of Hybrid Formation between Peptide Nucleic Acids and DNA by Fluorescence Polarization in the Presence of Polylysine
โ Scribed by Theo T. Nikiforov; Sang Jeong
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 80 KB
- Volume
- 275
- Category
- Article
- ISSN
- 0003-2697
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## Abstract The interaction of fluoroquinacrine, 3โfluoroโ7โchloroโ9โ(diethylaminoโ1โmethylbutylโamino)acridine, with poly(A), DNA, and tRNA has been investigated by monitoring changes in the ^19^Fโnmr properties, the fluorescence, and the optical absorbance of the drug. The changes in the properti