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Detection of human spermatid-specific transcripts in peripheral blood lymphocytes of males and females

✍ Scribed by Ryszard Slomski; Manfred Schloesser; Hanna Chlebowska; Jochen Reiss; Wolfgang Engel

Publisher
Springer
Year
1991
Tongue
English
Weight
560 KB
Volume
87
Category
Article
ISSN
0340-6717

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✦ Synopsis

We describe the detection of ectopic ("illegitimate") transcripts of the proacrosin and protamine 2 genes, which are specific for human spermatogenesis, in non-cultured peripheral blood lymphocytes. After specifically-primed reverse transcription of total lymphocyte RNA, these rare transcripts can be directly visualised after two rounds of polymerase chain reaction with nested primers. Sequence and restriction analyses of the corresponding fragments have confirmed that transcripts of proacrosin and protamine 2 are present in the lymphocytes not only of males, but also of adult females.

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