𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Detection of human herpesvirus-6 DNA by polymerase chain reaction in serum or plasma

✍ Scribed by Dr. Li-Min Huang; Pei-Fen Kuo; Chin-Yun Lee; Jen-Yang Chen; Mei-Ying Liu; Czau-Siung Yang

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
401 KB
Volume
38
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Human herpesvirus‐6 (HHV‐6) is a newly identified human pathogen. Currently clinicians rely mainly on blood lymphocyte culture and serological tests to diagnose HHV‐6 infection. The polymerase chain reaction (PCR) was carried out on the plasma or sera of patients to determine the value of PCR in the diagnosis of HHV‐6 infection. A total of 30 patients entered the study; 10 were experiencing acute HHV‐6 infections and 20 were healthy and served as controls. HHV‐6 DNA was detected by PCR in the serum or plasma of the 10 cases with acute HHV‐6 infections. All 20 controls had no HHV‐6 DNA in their sera. The time for serum to become PCR‐positive coincided with the appearance of IgG HHV‐6 antibody. The relatively late presence of HHV‐6 DNA in serum might result from late lysis of infected cells by immune responses. It is concluded that detection of HHV‐6 DNA by PCR in the serum is a valuable tool for the diagnosis of acute and/or active viral infection. Β© 1992 Wiley‐Liss, Inc.

πŸ“œ SIMILAR VOLUMES

Detection of human herpesvirus 6 DNA in
Detection of human herpesvirus 6 DNA in throat swabs by polymerase chain reaction
✍ Shinji Kido; Kazuhiro Kondo; Toshio Kondo; Tsuneo Morishima; Michiaki Takahashi; πŸ“‚ Article πŸ“… 1990 πŸ› John Wiley and Sons 🌐 English βš– 404 KB

## Abstract The detection of human herpesvirus 6 (HHV‐6) DNA was carried out in throat swabs of adults and children by the polymerase chain reaction, and isolation of virus was also attempted from peripheral mononuclear cells. Although virus was isolated only from peripheral blood mononuclear cells

Detection of HPV DNA in trichilemmomas b
Detection of HPV DNA in trichilemmomas by polymerase chain reaction
✍ Rohwedder, Angela; Keminer, Oliver; Hendricks, Carlo; Schaller, JΓΆrg πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 269 KB πŸ‘ 2 views

Paraffin sections of 11 formalin-fixed trichilemthe genital HPVs (A); the epidermodysplasia verrucimomas were investigated for the presence of formis (EV)-associated HPVs (B); ungulate associated human papillomavirus (HPV) DNA by the polypapillomaviruses (C and D) and cutaneous HPVs (E) merase chain

Detection of human herpesvirus 6 DNAs in
Detection of human herpesvirus 6 DNAs in samples from several body sites of patients with exanthem subitum and their mothers by polymerase chain reaction assay
✍ Sadao Suga; Takehiko Yazaki; Yqji Kajita; Takao Ozaki; Yoshizo Asano πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 492 KB

DNAs in peripheral blood mononuclear cells (MNCs), plasma, saliva, stool, and urine from three patients with exanthem subitum and in peripheral blood MNCs, plasma, and saliva from their mothers. HHV-6 DNAs were detected in MNCs during and after the disease and were found in plasma only in the acute

Correlation between the detection of vir
Correlation between the detection of viral DNA by the polymerase chain reaction in peripheral blood leukocytes and serological responses to human herpesvirus 6, human herpesvirus 7, and cytomegalovirus in renal allograft recipients
✍ Osman, H. K.; Peiris, J. S. M.; Taylor, C. E.; Karlberg, J. P. E.; Madeley, C. R πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 61 KB πŸ‘ 2 views

Diagnosis of significant infections by human herpesvirus 6 (HHV6) and 7 (HHV7) in transplant patients has proved difficult because both viruses are ubiquitous and can cause persistent infections in their hosts. The significance of viral DNA detected in peripheral blood leukocytes (PBLs; DNAemia) by