Detection of human blood by cloth-based enzyme immunoassay of immunoglobulin G
β Scribed by Yen Ly; Hiroshi Yamazaki
- Book ID
- 104647036
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 276 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0951-208X
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β¦ Synopsis
A specific and sensitive assay for the detection of human blood was developed using polyester cloth coated with goat anti-human IgG antibody to capture human IgG, an abundant and stable protein in blood. The captured IgG was detected by the reaction betweengoat anti-human IgG antibody-peroxidase conjugate and a chromogenicperoxidase substrate. Because the assay is simple and rapid, and permits simultaneous analysis of multiple samples, it has the potential to be used as a forensic test for human blood.
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