Detection of HTLV-I antigen in peripheral and cord blood lymphocytes from carrier mothers
β Scribed by Satow, Y.; Hashido, M.; Honda, H.; Mizuno, M.; Kawana, T.; Ishikawa, K.; Hayami, M.
- Book ID
- 123034702
- Publisher
- The Lancet
- Year
- 1991
- Tongue
- English
- Weight
- 287 KB
- Volume
- 338
- Category
- Article
- ISSN
- 0140-6736
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## Abstract The level of proviral DNA in peripheral blood mononuclear cells from a representative group of asymptomatic HTLVβI carriers in Miyazaki district, an HTLVβI endemic area in Japan, was determined by a singleβcycle polymerase chain reaction method (PCR). Of 217 subjects, 26% had a high lev
We screened 596 cord-blood samples by nested short PCRs for the gag and pX regions of HTLV-1 which are capable of detecting a single copy. The samples were derived from 449 and 147 babies born to seropositive and seronegative mothers respectively. Of these, 20 samples were positive in at least one o