The polymerase chain reaction (PCR) technique has been utilized for the detection of hepatitis B virus (HBV) DNA, and several factors related to the selection of primer pairs for the PCR amplification have been demonstrated. The sensitivity of the PCR assay was compared with that of slot-blot hybrid
Detection of histamine-producing bacteria using polymerase chain reaction techniques and DNA probes
β Scribed by Rita Alves; Ana dos Santos; Maria Martins
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 37 KB
- Volume
- 214
- Category
- Article
- ISSN
- 0044-3026
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This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set