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Detection of high-risk HPV types by the hybrid capture 2 test

✍ Scribed by George Terry; Linda Ho; Philip Londesborough; Jack Cuzick; Iwona Mielzynska-Lohnas; Attila Lorincz

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
178 KB
Volume
65
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

A hybrid capture test that can be used to detect at least 13 high‐risk HPV types (referred to collectively as HPV‐HR) in cervical scrapes (Hybrid Capture 2 probe set B, HC2‐B) was evaluated. The HC2‐B test is accurate and highly reproducible and the results obtained show excellent agreement with those obtained by a multiplexed type‐specific polymerase chain reaction (mts‐PCR). An additional assay to identify a subset of HPV‐HR types may improve specificity without compromising sensitivity in HC2‐B positive specimens with a test result close to the cut‐off value given by 1 pg/ml HPV DNA. J. Med. Virol. 65:155–162, 2001. © 2001 Wiley‐Liss, Inc.

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