Detection of heat shock proteins 70 in the gill, liver, and cardiac muscle of Carassius auratus with confocal microscopy
✍ Scribed by Dongwu Liu; Zhiwei Chen; Xuqian Zhou
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 874 KB
- Volume
- 75
- Category
- Article
- ISSN
- 1059-910X
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✦ Synopsis
Abstract
Heat shock proteins 70 (Hsp70) are the most extensively studied heat shock proteins for the cellular abundance and cytoprotective effects. Hsp70 induction and subsequent quantification has been used as a sensitive system for aquatic toxicity risk assessment. In this study, the confocal microscopy was used to localize Hsp70 in Carassius auratus (C. auratus) with immunohistochemical technology. There are different zooms to select to analyze the object at the same field of vision with one objective lens with confocal microscopy. It need not change objective lens to observe the details of tissues. In this study, the tissue slices of C. auratus were observed with the 20‐fold objective lens. Furthermore, the zooms of 1, 2, and 3 were used to acquire the distribution of Hsp70 in the tissue slices of C. auratus, and the clearer images of Hsp70 in the tissues were acquired. The results indicated that Hsp70 were present in the gill, liver, and cardiac muscle of C. auratus, and a method was established to detect Hsp70 in the tissues of C. auratus with confocal microscopy. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.