We developed a polymerase chain reaction assay for the direct detection of hepatitis B virus in paraffin-embedded liver tissue and applied this assay to determine whether hepatitis B virus DNA exists in livers with chronic hepatitis non-A, non-B. Fifty five liver biopsy samples were studied: 11 from
Detection of HBV DNA in non-A, non-B hepatic tissues using the polymerase chain reaction assay
β Scribed by Ohkoshi, Showgo
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 689 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0435-1339
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The polymerase chain reaction (PCR) technique has been utilized for the detection of hepatitis B virus (HBV) DNA, and several factors related to the selection of primer pairs for the PCR amplification have been demonstrated. The sensitivity of the PCR assay was compared with that of slot-blot hybrid
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