Detection of halogenated hydrocarbons by a microbial sensorsystem using a stop-flow-technique

✍ Scribed by J. Peter; W. Hutter; W. Stöllnberger; W. Hampel

• Book ID: 104648449
• Publisher: Springer-Verlag
• Year: 1996
• Tongue: English
• Weight: 389 KB
• Volume: 10
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00158943

✦ Synopsis

1995. a stop-flow-technique was developed for the determination of halogenated hydrocarbons in water samples. Cells of Rhodocuccus sp. DSM 6344 were imniobilized in alginate beads and placed in a stirred flow-through reactor. The time of incubation, the bacterial cell density and the amount of alginate beads in the reactor on the response of the system as determined by the drop in EMF was investigated. Optimal conditions were achieved with 2 g beads containing a bacterial cell concentration of 0,l g cells wet wt/g matrix and an incubation time of 20 min. Calibrations with chlorinated and brominated substrates like ethyl bromide, 1 ,Zdl&romopropane, isobutyl bromide, 1 -chlorobutane and 1,5dichloropentane showed a non-linear dependence at low substrate concentrations. The detection limits for ethyl bromide and l-chlorobutane were estimated as 0.02 mgA and 0.45 mg/l, respectively; the relative standard deviation was below 10 %. The great advantage of the stop-flow-technique compared to discontinuous measurements can be seen in a simplified handling and an increase of sample capacity.