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Detection of H-2 antigens on 8-cell mouse embryos

✍ Scribed by Cozad, Karen M. ;Warner, Carol M.

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
402 KB
Volume
221
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

H‐2 antigens were detected on 8‐cell mouse embryos of the b, k, and d haplotypes, but not on 8‐cell embryos of the a haplotype. the technique used for detection of H‐2 antigens on the embryos was a highly sensitive, automated, complement‐dependent cytotoxicity assay, based on the principle that live embryos will incorporate ^3^H‐thymidine into DNA, but embryos killed with antiserum and complement will not. The amount of H‐2 present on 8‐cell embryos is apparently less than that present on blastocysts. A possible function of H‐2 antigens in development is proposed.

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