✦ LIBER ✦
Detection of Giant Myofibrillar Proteins Connectin and Nebulin by Electrophoresis in 2% Polyacrylamide Slab Gels Strengthened with Agarose
✍ Scribed by R. Tatsumi; A. Hattori
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 422 KB
- Volume
- 224
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
We have established an improved method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis to facilitate analysis of giant myofibrillar proteins connectin and nebulin, whose molecular masses are about 3000 and (700 \mathrm{kDa}), respectively. This method consists of (2 %) polyacrylamide slab gels strengthened with agarose and a specific buffer system. Our (2 %) polyacrylamide slab gels are superior to usual 1.8 or (2 %) polyacrylamide disc gels in preparation, handling, and resolution. 1995 Academic Press, Inc.