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Detection of genes essential in specific niches by signature-tagged mutagenesis

✍ Scribed by Dario E Lehoux; Roger C Levesque

Book ID
104361517
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
246 KB
Volume
11
Category
Article
ISSN
0958-1669

No coin nor oath required. For personal study only.

✦ Synopsis

Variations of the signature-tagged mutagenesis (STM) technique are now possible and the method can be applied to most pathogens that have an STM-selectable phenotype in a host system. STM screening of 15,040 mutants from 11 bacterial species identified 323 in vivo attenuated mutants. As a genome-scanning tool, STM will yield information about genes with unknown functions as well as information crucial for understanding microbial pathogenesis.

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