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Detection of Gastric Cancer Micrometastases in Lymph Nodes by Amplification of Keratin 19 mRNA with Reverse Transcriptase-Polymerase Chain Reaction

✍ Scribed by Noguchi, Sbinzaburo ;Hiratsuka, Masahiro ;Furukawa, Hiroshi ;Aihara, Tomohiko ;Kasugai, Tsutomu ;Tamura, Sumihito ;Imaoka, Shingi ;Koyama, Hiroki ;Iwanaga, Takeshi


Book ID
108581221
Publisher
Wiley (Blackwell Publishing)
Year
1996
Tongue
English
Weight
324 KB
Volume
87
Category
Article
ISSN
0910-5050

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Reverse transcriptase-polymerase chain reaction (RT-PCR) targeted at keratin 19 mRNA was applied to detect circulating cancer cells in the peripheral and portal blood of pancreatic and gastric cancer patients. Keratin 19 mRNA expression was studied by RT-PCR in cancer tissues (12 pancreatic and 15 g