Detection of Gastric Cancer Micrometastases in Lymph Nodes by Amplification of Keratin 19 mRNA with Reverse Transcriptase-Polymerase Chain Reaction
β Scribed by Noguchi, Sbinzaburo ;Hiratsuka, Masahiro ;Furukawa, Hiroshi ;Aihara, Tomohiko ;Kasugai, Tsutomu ;Tamura, Sumihito ;Imaoka, Shingi ;Koyama, Hiroki ;Iwanaga, Takeshi
- Book ID
- 108581221
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 1996
- Tongue
- English
- Weight
- 324 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0910-5050
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BACKGROUND. Amplification of keratin 19 mRNA (K19) by reverse transcriptasepolymerase chain reaction (RT-PCR) has been shown to be a sensitive method to detect occult breast cancer metastases in lymph nodes. METHODS. Axillary lymph nodes were obtained from 126 patients with breast cancer, and metast
Reverse transcriptase-polymerase chain reaction (RT-PCR) targeted at keratin 19 mRNA was applied to detect circulating cancer cells in the peripheral and portal blood of pancreatic and gastric cancer patients. Keratin 19 mRNA expression was studied by RT-PCR in cancer tissues (12 pancreatic and 15 g