✦ LIBER ✦

Detection of functional groups and antibodies on microfabricated surfaces by confocal microscopy

✍ Scribed by Amir H. Nashat; Mario Moronne; Mauro Ferrari

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 305 KB
Volume: 60
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19981020)60:2<137::aid-bit1>3.0.co;2-o

No coin nor oath required. For personal study only.

✦ Synopsis

Fluorescence confocal microscopy was used to characterize micron-sized microfabricated silicon particles and planar oxide surfaces after silanization and immobilization of IgG antibody. Surfaces treated with amino-and mercaptosilanes were tested for the presence of amine and sulfhydryl groups by labeling with specific fluorescein probes. In addition, human antibody (IgG) was immobilized to the thiol-coated microparticles using the heterobifunctional crosslinker succinimidyl 4-(N-maleimidolmethyl)-cyclohexane-1-carboxylate. Estimates of the surface density of IgG were consistent with 8.3% of a monolayer of covalently-bound antibody. Confocal images confirmed uniform layers of both silanes and antibodies on the microparticles. The sensitivity limit for the confocal measurements was determined to be as low as 1.5 × 10 -5 fluors per nm 2 .

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