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Detection of Escherichia coliα-haemolysin genes and their expression in a human faecal strain of Enterobacter cloacae

✍ Scribed by Javier Prada; Lothar Beutin


Book ID
109316966
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
361 KB
Volume
79
Category
Article
ISSN
0378-1097

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A 6.4 Kb HindIII fragment of Bacillus stearothermophilus DY-5 DNA cloned in Escherichia coli using pBR322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. In attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 Kb HindIII