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Detection of endometrial cancer by flow cytometry using two monoclonal antibodies

โœ Scribed by Koji Kobiki; Katsumi Tsukasaki; Kaneyuki Kubushiro; Yoichi Kobayashi; Kiyoshi Hasegawa; Takeshi Fukuchi; Koji Banno; Shiro Nozawa

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
167 KB
Volume
36
Category
Article
ISSN
0196-4763

No coin nor oath required. For personal study only.

โœฆ Synopsis

Background: To develop a supplementary diagnostic method for endometrial cancer by measuring the reactivity of various endometrial lesions with two monoclonal antibodies. Methods: We investigated the reactivity of various endometrial lesions with two monoclonal antibodies (MSN-1 and MSN-3) by flow cytometry (one-color and two-color methods).

Results:

The two-color method appeared to be suitable for use in place of simultaneous performance of the one-color methods with MSN-1 and MSN-3. The positivity rate for normal endometrium was 16.0% with the twocolor method, which was lower than the rate of 30.0% obtained with concomitant used of the one-color meth-ods. The positivity rate for endometrial cancer was high, 84.0%, with the two-color method. The positivity rate was 85.7% for well-differentiated endometrial cancer, 71.4% for moderately differentiated cancer, and 100.0% for poorly differentiated cancer; thus, the rate was high irrespective of the cellular differentiation. Conclusions: The two-color method is more useful than the one-color method as a supplementary diagnostic procedure for endometrial cancer. Cytometry 36: 150-156, 1999. 1999 Wiley-Liss, Inc.

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