Detection of endogenous and antibody-conjugated alkaline phosphatase with ELF-97 phosphate in multicolor flow cytometry applications

Detection of endogenous alkaline phospha

Detection of endogenous alkaline phosphatase activity in intact cells by flow cytometry using the fluorogenic ELF-97 phosphatase substrate

✍ William G. Telford; William G. Cox; Dalina Stiner; Victoria L. Singer; Stephen B 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 371 KB 👁 2 views

Background: The alkaline phosphatase (AP) substrate 2-(5Ј-chloro-2Ј-phosphoryloxyphenyl)-6-chloro-4-(3H)-quinazolinone (ELF -97 for enzyme-labeled fluorescence) has been found useful for the histochemical detection of endogenous AP activity and AP-tagged proteins and oligonucleotide probes. In this