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Detection of elastase activity with a zymogram method after isoelectric focusing in polyacrylamide gel

✍ Scribed by Concetta Gardi; Giuseppe Lungarella


Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
891 KB
Volume
140
Category
Article
ISSN
0003-2697

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A chromogenic substrate autography is described which allows characterization and quantification of serine proteases in crude systems after sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectric focusing. Separation of samples containing proteases by either method is followed by an