✦ LIBER ✦

Detection of early hemagglutination inhibitory antibodies to rubella virus by pretreatment of sera with streptococcal cells

✍ Scribed by Kimiko Kawano; Seigo Yamamoto; Yoichi Minamishima

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 599 KB
Volume: 19
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890190202

No coin nor oath required. For personal study only.

✦ Synopsis

A technique that is based on absorption of sera with streptococcal cells and hemagglutination inhibition (HI) was evaluated for its feasibility for serologic diagnosis of recent rubella. The mixture of AR1 and AW43 cells removes IgG and IgA from the kaolin-treated sera, leaving IgM and a trace of IgA, probably oligomeric IgA. Consequently, after the absorption with streptococci, the HI antibodies are detectable exclusively in the early sera of patients with rubella. The streptococci (AR1 and AW43) have several advantages as the absorbent over the staphylucoccus (Cowan I) that has been used routinely.

📜 SIMILAR VOLUMES

Detection of early antigens in nuclei of

Detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their specificity

✍ G. Giraldo; E. Beth; U. Hämmerling; G. Tarro; F. M. Kourilsky 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 975 KB

## Abstract Skin fibroblasts exposed to cytosine arabinoside (Ara C) were infected with either cytomegalovirus (CMV) or herpes simplex virus (HSV) type 1 and 2. Herpesvirus‐determined early antigens (HV‐EA), detected by anti‐complement immunofluorescence (ACIF), occurred primarily in the nuclei, an