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Detection of differentially expressed genes in Methylnitrosourea-induced rat mammary adenocarcinomas

✍ Scribed by Hu, Lan; Lin, Lin; Crist, Keith A.; Kelloff, Gary J.; Steele, Vernon E.; Lubet, Ronald A.; You, Ming; Wang, Yian

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 187 KB
Volume: 67
Category: Article
ISSN: 0730-2312
DOI: 10.1002/(sici)1097-4644(1997)28/29+<117::aid-jcb13>3.0.co;2-e

No coin nor oath required. For personal study only.

✦ Synopsis

In this study, altered gene expression in five methylnitrosourea (MNU)-induced rat mammary adenocarcinomas was investigated using a newly developed competitive cDNA library screening assay. In order to detect the differentially expressed cDNA transcripts, three cDNA libraries (rat mammary, rat liver, and rat kidney) with over 18,000 clones were differentially screened with competing normal and neoplastic mammary cDNA probes. Ninety-eight clones indicated by competitive hybridization to be differentially expressed in tumors were verified by dot-blot hybridization analysis. Of these clones, 45 were found to be overexpressed while 53 were underexpressed in tumors. Forty-five of the confirmed clones were further analyzed by single-pass cDNA sequence determination. Four clones showed homology with cytochrome oxidase subunit I, polyoma virus PTA noncoding region, cytoplasmic beta-actin, and mouse secretory protein containing thrombospondin motifs. Further investigation into the potential roles of these identified genes should contribute significantly to our understanding of the molecular mechanism(s) of rat mammary tumorigenesis.

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