Detection of different states of immunity to colon cancer in rats by a leukocyte adherence inhibition (LAI) assay assessing the adherence of T lymphocytes and monocytes selectively
✍ Scribed by Dr. Toshio Morizane; Hans Olov Sjögren
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- French
- Weight
- 400 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
Peripheral blood mononuclear cells (MNC) of rats, which had received a subcutaneous inoculation of either viable or X‐irradiated syngeneic DMH‐W49 colon carcinoma cells, were investigated sequentially for sensitization against tumor‐associated antigens by a new micro‐glass‐tube leukocyte adherence inhibition (LAI) assay. The number of adherent cells of MNC subpopulations was estimated by a cellular radioimmunoassay (CRIA), which utilizes anti‐monocyte (MC) antiserum or anti‐T‐cell monoclonal antibody (McAb) and ^125^I‐labelled protein A. LAI reactivity was demonstrated in rats sensitized with X‐irradiated tumor cells when assessing the adherence of both T lymphocytes and monocytes. It was found that reactivity with the two different types of indicator cells did not usually coincide in time. The same phenomenon was also observed in tumor‐bearing rats although with lower levels of LAI reactivity. In both groups the LAI response detected by anti‐T‐cell reagent mostly appeared earlier after tumor‐cell inoculation than the response demonstrated by anti‐MC serum. The T‐lymphocyte‐associated reactivity also disappeared more rapidly. These results suggest that different states of immune reactivity are reflected in LAI responses involving T lymphocytes and monocytes as indicator cells.