Detection of developmentally regulated genes of the myxobacteriumStigmatella aurantiacawith the transposon Tn5lacZ
✍ Scribed by Andreas Pospiech; Björn Neumann; Barbara Silakowki; Hans Ulrich Schairer
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 709 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0302-8933
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Mutants of a specialized lambda dmet transducing phage bearing the metJBLF gene cluster of Escherichia coli K12 were constructed using transposon Tn5. Two of these mutants, lambda dmet128::Tn5, MW77 and lambda dmet128::Tn5, 3-1, were used to locate precisely as well as confirm the existence of the m
Three new genes nifM, nifI and nifN have been mapped in the nif gene cluster of Klebsiella pneumoniae and a fourth gene nifJ has been confirmed as being a separate cistron. Polar nif mutations were obtained by transposition of Tn7 to plasmid pRD1, and of Tn5 and Tn10 to plasmid pMF100, a derivative