We developed a polymerase chain reaction assay for the direct detection of hepatitis B virus in paraffin-embedded liver tissue and applied this assay to determine whether hepatitis B virus DNA exists in livers with chronic hepatitis non-A, non-B. Fifty five liver biopsy samples were studied: 11 from
✦ LIBER ✦
Detection of cutaneous Leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction
✍ Scribed by Tamás Laskay; Tivadar L. Mikó; Yohannes Negesse; Werner Solbach; Martin Röllinghoff; Dominique Frommel
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 834 KB
- Volume
- 89
- Category
- Article
- ISSN
- 0035-9203
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Three oligonucleotide primer combinations selected from the 5' noncoding, the nucleocapsid and the putative nonstructural regions of the hepatitis C virus genome were compared in a nested polymerase chain reaction assay with respect to sensitivity and specificity for the detection of viral RNA in ch