Detection of Cucurbit yellow stunting disorder virus in Cucurbit Leaves Using Sap Extracts and Real-time TaqMan® Reverse Transcription (RT) Polymerase Chain Reaction (PCR)

Increased detection of rotavirus using a

Increased detection of rotavirus using a real time reverse transcription-polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhea

✍ Xiaoli L. Pang; Bonita Lee; Nasim Boroumand; Barbara Leblanc; Jutta K. Preiksait 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 118 KB

## Abstract Six‐hundred and twenty‐six stool specimens collected from children with diarrhea over a 12‐month period were tested for rotavirus using a real time reverse transcription‐polymerase chain reaction (RT‐PCR) assay, a conventional nested PCR assay and by electron microscopy (EM). A fragment