Detection of complement activation in human serum using C5S-Induced chemiluminescence of human granulocytes

✍ Scribed by A. Kapp; F. E. Maly; E. Schöpf

• Book ID: 104763707
• Publisher: Springer-Verlag
• Year: 1985
• Tongue: English
• Weight: 310 KB
• Volume: 278
• Category: Article
• ISSN: 0340-3696
• DOI: 10.1007/bf00412494

✦ Synopsis

The complement system can be activated by many factors, including immune complexes, leading to the generation of biologically active split products like C5a anaphylatoxin. This study presents a technique which may be used for measuring C5a activity in human serum. The tetanus-anti-tetanus immune complex and aggregated human IgG were used as model activators of the complement cascade. The C5a activity was measured by C5a-induced chemiluminescence of granulocytes; furthermore, a radioimmunoassay was used to detect the C5a peptide. There was a strongly positive correlation between the two assay systems. The described method should be useful as an alternative means of detecting complement activation in the serum of patients with inflammatory diseases.