Detection of CMV-matrix pp65 antigen in leucocytes by immunofluoreseence as a marker of CMV disease

✍ Scribed by Anneka Ehrnst; Lisbeth Barkholt; Christina Brattström; Jan Czajkowski; Otilia Teodosiu; Jan Tollemar; Per Ljungman

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 665 KB
• Volume: 39
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890390207

✦ Synopsis

The finding that active cytomegalovirus (CMV) infection is associated with an increased mortality after organ transplantation and the introduction of successful antiviral treatment render more urgent the development of rapid and accurate diagnostic methods. Rapid immunodiagnosis of active CMV infection was investigated by means of immunofluorescence staining of leucocytes by monoclonal antibodies. Monoclonals directed against the matrix protein, pp65, and ClonabR monoclonal antibody, which has been claimed to be directed against immediate early antigens, were used. All monoclonal antibodies directed against the matrix protein reacted equally well in staining of polymorphonuclear cells. Monoclonals described by Gerna et al.