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Detection of chrysotile asbestos by using a chrysotile-binding protein

✍ Scribed by Akio Kuroda; Tomoki Nishimura; Takenori Ishida; Ryuichi Hirota; Kazutaka Nomura

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
192 KB
Volume
99
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

In the current studies, we found that the DksA protein from Escherichia coli binds strongly to chrysotile, which is the most commonly used form of asbestos. We developed a convenient colorimetric assay for chrysotile using a fusion of DksA and alkaline phosphatase along with 5‐bromo‐4‐chloro‐3‐indolyl‐phosphate and nitro blue tetrazolium as substrates. Also, using a fusion of DksA and green‐fluorescent protein, we were able to detect chrysotile by fluorescence microscopy. Biotechnol. Bioeng. 2008;99: 285–289. Β© 2007 Wiley Periodicals, Inc.

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